Changes in redox status associated with polymorphisms of the glutathione S-transferase gene in women with a family history of breast cancer

Authors

  • Gretel Riverón Forment Centro Nacional de Genética Médica
  • Claudia Pérez López Centro Nacional de Genética Médica
  • Martha Sonia Robaina Castellanos Instituto Nacional de Oncología y Radiobiología
  • Marisol Peña Sánchez Instituto de Neurología y Neurocirugía
  • Reinaldo Gutiérrez Gutiérrez Centro Nacional de Genética Médica

Keywords:

breast cancer, family history, oxidative stress, GST genotypes, glutathione peroxidase, malonyldialdehyde.

Abstract

Introduction: The involvement of cellular redox status and antioxidant mechanisms is related to several of the risk factors for breast cancer.

Objectives: To determine the association between oxidative stress markers and glutathione S-transferase gene polymorphisms in women with a family history of breast cancer.

Methods: A case control-study was carried out. A total of 81 females were included, 47 of them with family antecedents of I and II grade of breast cancer and 34 as controls. They were determined: plasma concentrations of malonildialdehyde, advanced products of protein oxidation, enzymatic activities of Cu-Zn superoxide dismutase, catalase and glutathione peroxidase by spectrophotometric methods. In addition, the presence of the homozygous deletion of the GSTM1 and GSTT1 genes was detected by multiplex PCR.

Results: The women with a family history of breast cancer showed an increase in the concentrations of malonildialdehyde together with a high activity of the glutathione peroxidase with respect to the control group. 53.1% and 12.5% of the women with a family history had the homozygous deletion of the GSTM1 and T1 genes, respectively. The combination of the functional variants M1+/T1+ was related to an increase in the enzyme antioxidant systems, while the combination M1*0/T1+ was associated with the increase in the concentrations of non-enzymatic antioxidants in the plasma.

Conclusions: Malonildialdehyde levels and Glutathione peroxidase activity could be considered risk factors in women with a family history. The combinations of the variants of the GST genes are related to the activation of compensatory antioxidant mechanisms.

 

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Author Biography

Gretel Riverón Forment, Centro Nacional de Genética Médica

Investigador Auxiliar, Profesor Asistente. Departamento de Genética Bioquímica.

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Published

2022-04-12

How to Cite

1.
Riverón Forment G, Pérez López C, Robaina Castellanos MS, Peña Sánchez M, Gutiérrez Gutiérrez R. Changes in redox status associated with polymorphisms of the glutathione S-transferase gene in women with a family history of breast cancer. revgencom [Internet]. 2022 Apr. 12 [cited 2025 Jun. 2];13(2). Available from: https://revgenetica.sld.cu/index.php/gen/article/view/117

Issue

Vol. 13 No. 2 (2020): MAYO - AGOSTO

Section

Artículos originales

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