Evaluation of the Quality Control of Hemoglobin Electrophoresis in the Hemoglobinopathies Program (2009-2019)

Authors

  • Yadira Valdés Fraser Centro Nacional de Genética Médica. La Habana https://orcid.org/0000-0002-2585-3208
  • Alina Concepción Álvarez Centro Nacional de Genética Médica. La Habana
  • Tatiana Acosta Sánchez Centro Nacional de Genética Médica. La Habana
  • Sandra Bárbara Correa Jiménez Centro Nacional de Genética Médica. La Habana

Keywords:

quality control, electrophoresis, hemoglobinopathies.

Abstract

Introduction: The National Center for Medical Genetics implemented the external quality control of hemoglobin electrophoresis in the hemoglobinopathy program in the National Genetics Network in 2009. The implementation of this control leads to evaluating the analytical accuracy of the laboratory, comparing results obtained from the same sample between the reference laboratory and the provincial laboratories.

Objective: To describe the results of the external quality control of hemoglobin electrophoresis and the frequency of genotypic variants in the hemoglobinopathy program, period 2009 - 2019.

Methods: A descriptive and cross-sectional observational study was carried out, using statistical data from the Quality Control Registry. Random samples (5 and 15) of blood with EDTA were processed monthly by province. The HYDRASYS 2 equipment was used in the analysis of hemoglobin variants and thalassemias. The evaluation of the program was performed: rejected samples (pre-analytical phase), processed and positive samples (analytical phase), discrepant samples in the results (post-analytical phase).

Results: 10 370 controls were received, 10 090 were processed and 2.8% of the samples were rejected. In 3477 controls, a variation in the structure, quantity and / or function of the hemoglobin molecule was observed. 1.2% of the analyzed samples presented discrepancies in the results.

Conclusions: The electrophoresis EQC showed satisfactory results, despite the fact that some evaluated laboratories do not comply with all the procedures and criteria of the phases evaluated in the diagnosis of hemoglobinopathy. It is confirmed that the most frequent genotypic variant in Cuba is AS hemoglobinopathy.


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Author Biography

Yadira Valdés Fraser, Centro Nacional de Genética Médica. La Habana

Laboratorio de Genética Bioquimica

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Published

2022-10-05

How to Cite

1.
Valdés Fraser Y, Concepción Álvarez A, Acosta Sánchez T, Correa Jiménez SB. Evaluation of the Quality Control of Hemoglobin Electrophoresis in the Hemoglobinopathies Program (2009-2019). revgencom [Internet]. 2022 Oct. 5 [cited 2025 Jul. 15];14(1). Available from: https://revgenetica.sld.cu/index.php/gen/article/view/170

Issue

Vol. 14 No. 1 (2022): ENERO - AGOSTO

Section

Artículos originales

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